Possible Protective Effect of Ascorbic Acid on the Histopathological Changes of Acrylamide on Jejunum of Adult Male Albino Rats

Mohammed, Doha; Nor _Eldin, Eman; Khalifa, Asmahan

doi:10.21608/smj.2017.39250

Possible Protective Effect of Ascorbic Acid on the Histopathological Changes of Acrylamide on Jejunum of Adult Male Albino Rats

Document Type : Original Article

Authors

10.21608/smj.2017.39250

Abstract

Introduction: Acrylamide (ACR) is a water soluble vinyl monomer that has multiple chemical and industrial applications. Several studies were used with liver, kidney, brain, erythrocyte and proved that (ACR) increase glutathione-s-transferase   and causing oxidative stress. Jejunum is the most sensitive part of the intestine, and most of the absorption process occurred in it.   Ascorbic acid is natural antioxidant that prevents the increased production of free radicals induced by oxidative damage to various cellular components.
Aim of the work: to study the effect of ACR on the jejunum of adult male albino rats and to evaluate the possible role of ascorbic acid as a protective agent.
Material and methods: A thirty albino rats were divided into 3 groups:
Group Ι: were served as untreated control group.
Group IIa received ACR 30 ml\kg b.w orally dailly for 28days
Group IIb: received both ACR 30 ml\kg b.w orally simultaneously with ascorbic acid 400ml \kg.b.w intraperitoneally daily for 28 day.
Results
Exposure to ACR led to histological changes in Group IIa in the form of widening and shortening of villi compared to control group sections. There was inflammatory cell infiltration in the lamina propria,
Group IIb showed an improvement in comparison with a groupIIa. This improvement include restoration of villous shape and length, less inflammatory cells infiltration
Conclusion: ACR led to histopathological changes in jejunum. These changes attenuated by simultaneous administration of ascorbic acid with ACR.

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