MOLECULAR GENETIC AND LABORATORY FINDINGS IN INFERTILE MEN WITH NON-OBSTRUCTIVE AZOOSPERMIA

Shaeer, Kamal; NADA, ESSAMELDIN ABDELAZIZ; Abdo, Ramadan Saleh; El Hamshary, Manal; Abd El-Latif, Ashraf Ahmed

doi:10.21608/smj.2017.36673

MOLECULAR GENETIC AND LABORATORY FINDINGS IN INFERTILE MEN WITH NON-OBSTRUCTIVE AZOOSPERMIA

Document Type : Original Article

Authors

¹ Department , of Andrology, Sexology and STIs- Faculty of Medicine- Cairo University

² DERMATOLOGY, Venereology and Andrology, Faculty of Medicine , sohag university

³ Department , of Dermatology, Venereology and Andrology , Faculty of Medicine- , Sohag University

⁴ Department , of Molecular Diagnostics and Therapeutics- Genetic Engineering and Biotechnology Research Institute- Sadat City University.

⁵ Department of Dermatology, Venereology and Andrology- Faculty of Medicine- Sohag University.

10.21608/smj.2017.36673

Abstract

Objectives:To study therelationship between Y-chromosome microdeletions withclinicaland laboratory findings in infertile men with non-obstructive azoospermia (NOA).
Design: Cross-sectionalstudy.
Patients: Infertile men withnon-obstructive azoospermia (n = 146).
Methods: Clinical evaluation and scrotal colour Doppler ultrasonography were evaluated. Standard semen analysis andserum levels of hormones(FSH, LH, total testosterone and prolactin) were performed. Multiplex PCR was done for detection of Y chromosome microdeletions.
Results:AZF deletions were detected in 9.59% of azoospermic men.CompleteAZFc was detected in 2.05% of azoospermic men.Partial AZFc deletions were found in 7.5% of azoospermic men, with gr/gr deletion in 6.85% and b2/b3 deletion in 0.69%.There was no significant difference between patients with AZF deletions and azoospermic men without deletions as regards testicular volume and serum levels of reproductive hormones.
Conclusions:Microdeletions of Y chromosome may play a role in pathogenesis of NOA. The testicular volumes as well as levels of reproductive hormoneswere not correlated with the finding of Y chromosome microdeletions.

Keywords

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