COMPARATIVE RESULTS OF RAPD-PCR GENETIC POLYMORPHISM OF SCHISTOSOMA HAEMATOBIUM

Document Type : Original Article

Authors

1 Medical parasitology department, Sohag Faculty of Medicine, Sohag University

2 Medical Parasitology Department, Sohag Faculty of Medicine

3 Department of Medical Parasitology, Sohag Faculty of Medicine, Sohag University. Egypt

4 Department of Medical Parasitology, Sohag Faculty of Medicine, Sohag University.

Abstract

Schistosomiasis is one of the public health threats in Egypt. S. hematobium infection significantly increases the risk of urinary bladder metaplasia and cancer. The study of the genetic diversity of S. hematobioum might help predict the severity of the disease and the resistance to treatment. In the present study, the DNA of the S. hematobium was amplified using RAPD-PCR from 27 urine samples and in addition, a positive control sample was used. We used RAPD-PCR primers (A01, A02, A12, A13, and Y20). DNA electrophoresis was done on a 1.2% agarose gel and samples were run with high molecular weight and a low-molecular weight100pb ladder. The samples generated a total of 182 bands with 5 primers. The average band numbers were 3 with primer A01, 2 with primer A02, 1 with primer A12, 3 with primer 3, and 2 with primer Y20. The average molecular weight for the generated bands with each primer was 938pb, 738pb, 1425pb, 1006pb, and 866pb respectively. Our results show genetic polymorphism in the samples specifically in primers A02, A13, and Y20. This genetic polymorphism was evident by the differences in the band numbers and weights (allelic composition). Further research is required to assess the relationship between genetic polymorphism and pathology caused by S. hematobium infection and finally the response to treatment.

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