N-acetyl-L-Cysteine ameliorates oxidative stress and reproductive toxicity induced by nicotine in male rats

Document Type : Original Article

Authors

1 Department of Pharmacology, Faculty of medicine, Sohag University

2 Department of Pharmacology, Faculty of medicine, Assiut University

Abstract

Background: The current study assessed the protective role of N-acetyl-L-Cysteine in alleviating the detrimental effect of nicotine on reproductive functions in male rats.
Methods: twenty four adult male albino rats were divided into four groups of six rats. Control group was treated orally with 0.5ml/kg normal saline, nicotine treated group received 1.0 mg/kg of nicotine i.p, N-acetyl-L-Cysteine treated group received 100 mg/kg and 200 mg/kg i.p for four weeks.
Results: Nicotine caused significant reduction in serum level of total antioxidant, testosterone and luteinizing hormone. It also caused a significant reduction in sperm count. There was impairment in testicular histology of rats treated with nicotine. N-acetyl-L-Cysteine improved the reduction in sperm count, hormone levels and testicular alterations observed in nicotine treated rats.
Conclusion: The study shows that nicotine exerts significant deleterious effects on male reproductive system and the concurrent administration of N-acetyl-L-Cysteine ameliorated these detrimental effects.

Keywords


  1. Ahmed F.E.-K., Mohamed Abdo Nassan, Ahmed Abdel Aziz Baiomy, Tamer Ahmed Ismail, Soliman M.M., Hossam Fouad Attia. (2014) potential ameliorative role of N-acetylcysteine against testicular dysfunction Induced by titanium dioxide in male albino rats. American Journal of Pharmacology and Toxicology 9:29-38.
  2. Blanco M.J., M. L., C. A.-G. (2012) Effect of current tobacco on the male reproductive hormone profile  Sci Total Environ 426:100-105.
  3. Dodd S., Dean O., Copolov D.L., Malhi G.S., Berk M. (2008) N-acetylcysteine for antioxidant therapy: pharmacology and clinical utility. Expert Opin Biol Ther 8:1955-1962. DOI: 10.1517/14728220802517901.
  4. El-Maddawy Z.K., El-Sayed Y.S. (2018) Comparative analysis of the protective effects of curcumin and N-acetyl cysteine against paracetamol-induced hepatic, renal, and testicular toxicity in Wistar rats. Environ Sci Pollut Res Int 25:3468-3479. DOI: 10.1007/s11356-017-0750-0753.
  5. Fairuz A., Hashida  N, M. M. (2011) Effect  of  nicotine  and  goat  milk coadministration  on  rat  testis and sperm parameters. . Austral J Basic Appl Sci 5(12):2738–2741. 5:2738–2741.
  6. Funabashi T., Sano A., Mitsushima D., Kimura F. (2005) Nicotine inhibits pulsatile luteinizing hormone secretion in human males but not in human females, and tolerance to this nicotine effect is lost within one week of quitting smoking. J Clin Endocrinol Metab 90:3908-13. DOI: 10.1210/jc.2005-0041
  7. Grief S.N. (2011) Nicotine dependence: health consequences, smoking cessation therapies  and pharmacotherapy.Prim Care 38:23-39, v. DOI: 10.1016/ j. pop. 2010. 11.003.
  8. Heidary F., R A., A L. (2012) The Effects of Cigarette  or  Hookah  Smoking on  Serum Levels  of LH,  FSH  or  Testosterone  in  Male  Rats.. nternational Conference  on  Medical,  Biological  and Pharmaceutical Sciences. .
  9. Hruskovicova H., Duskova M., Simunkova K., Hill M., Pospisilova H., Racz B., Kralikova E., Vondra K., Starka L. (2013) Effects of smoking cessation on hormonal levels in men. Physiol Res 62:67-73.
  10. Jana K., Samanta PK, DK. D. (2010) Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. . Toxicol Sci 116: 647–659.
  11. Kavitharaj N.K., Vijayammal P.L. (1999) Nicotine administration induced changes in the gonadal functions in male rats. Pharmacology 58:2-7. DOI: 10.1159/000028262.
  12. Kumamoto M., Sonda T., Nagayama K., Tabata M. (2001) Effects of pH and metal ions on antioxidative activities of catechins. Biosci Biotechnol Biochem 65:126-32. DOI: 10.1271/bbb.65.126.
  13. Mlynarcikova A., Fickova M., Scsukova S. (2005) Ovarian intrafollicular processes as a target for cigarette smoke components and selected environmental reproductive disruptors. Endocr Regul 39:21-32.
  14. Mosbah R., Yousef M.I., Mantovani A. (2015) Nicotine-induced reproductive toxicity, oxidative damage, histological changes and haematotoxicity in male rats: the protective effects of green tea extract. Exp Toxicol Pathol 67:253-9. DOI: 10.1016/j.etp.2015.01.001.
  15. Nashwa A.-A.A., A. A. Kawkab, S. M. Mouneir. (2011) The Protective effect of Ginger and N- Acetyl Cysteine on Ciprofloxacin-Induced Reproductive Toxicity in Male Rats. Journal of American Science 7:741-752.
  16. Oyeyipo I.P., Raji Y., Bolarinwa A.F. (2013) Nicotine alters male reproductive hormones in male albino rats: The role of cessation. J Hum Reprod Sci 6:40-44. DOI: 10.4103/0974-1208.112380.
  17. Oyeyipo I.P., Raji Y., Bolarinwa A.F. (2014) Nicotine alters serum antioxidant profile in male albino rats. N Am J Med Sci 6:168-71. DOI: 10.4103/1947-2714.131240.
  18. Ramlau-Hansen C.H., Thulstrup A.M., Aggerholm A.S., Jensen M.S., Toft G., Bonde J.P. (2007) Is smoking a risk factor for decreased semen quality? A cross-sectional analysis. Hum Reprod 22:188-196. DOI: 10.1093/humrep/del364.
  19. Reddy P.S., Rani G.P., Sainath S.B., Meena R., Supriya C. (2011) Protective effects of N-acetylcysteine against arsenic-induced oxidative stress and reprotoxicity in male mice. J Trace Elem Med Biol 25:247-53. DOI: 10.1016/j.jtemb.2011.08.145.
  20. Sankako M., Garcia PC, Piffer RC, OM. P. (2013) Semen and reproductive parametersduring some abstinence periods after cigarette smoke exposure in male rats. Braz Arch Biol Technol 56:93-100.
  21. Seema P., Swathy S.S., Indira M. (2007) Protective effect of selenium on nicotine-induced testicular toxicity in rats. Biol Trace Elem Res 120:212-8. DOI: 10.1007/s12011-007-8021-7.
  22. Sen C., Packer L. (200) Role of glutathione on antioxidant redox systems. . Am J Clin Nutr 72 653S–669S.
  23. Siti B.B., Kho J.H., Lee J.H., amaLingam A.R., Fatin F aRhana Jubaidi, eLda SuRhaida Latif, SatiRah Zainalabidin, izatuS Shima Taib, JamaLudin, Nassan M.A. (2017) Low-dose Nicotine Exposure Induced the Oxidative Damage of Reproductive Organs and Altered the Sperm Characteristics of Adolescent Male Rats. Malays J Med Sci 2450-2457.
  24. Tweed J.O., Hsia S.H., Lutfy K., Friedman T.C. (2012) The endocrine effects of nicotine and cigarette smoke. Trends Endocrinol Metab 23:334-42. DOI: 10.1016/j.tem.2012.03.00.
  25. Walczak-Jedrzejowska R., Wolski J.K., Slowikowska-Hilczer J. (2013) The role of oxidative stress and antioxidants in male fertility. Cent European J Urol 66:60-7. DOI: 10.5173/ceju.2013.01.
  26. Yoshida M., Kitani T., Takenaka A., Kudoh K., Katsuda S.I., Taya K., Kurokawa Y., Maekawa A. (2002) Lack of effects of oxolinic acid on spermatogenesis in young adult and aged Wistar rats. Food Chem Toxicol 40:1815-1825.